ELISA/assay

Mouse High mobility group protein B1 ELISA Kit

MBS762757

48-Strip-Wells

295 USD

ELISA Kit

Mouse High mobility group protein B1 ELISA Kit

High mobility group protein B1

Hmgb1/HMG-1/Amphoterin/high mobility group box 1/High mobility group protein 1/high-mobility group (nonhistone chromosomal) protein 1/high-mobility group box 1/HMG-1/HMG1DKFZp686A04236/HMG3/SBP-1/Sulfoglucuronyl carbohydrate binding protein

high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; high mobility group box 1; High mobility group protein 1; HMG-1

Hmgb1

HMGB1; HMGB1; HMG1; HMG3; HMG-1; SBP-1; HMG1; HMG-1

HMGB1_HUMAN

Mouse

215

This assay has high sensitivity and excellent specificity for detection of Hmgb1 . No significant cross-reactivity or interference between Hmgb1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/ml. Sensitivity: < 9.375pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hmgb1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hmgb1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Hmgb1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Hmgb1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hmgb1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hmgb1 can be calculated.

926459557

NP_001300822.1

NM_001313893.1

24,894 Da

Activated TLR4 Signalling Pathway (1269236); Activation Of DNA Fragmentation Factor Pathway (1270293); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1269258); Apoptosis Pathway (1270262); Apoptosis Induced DNA Fragmentation Pathway (1270292); Apoptotic Execution Phase Pathway (1270287); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276)

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Protein type: Nuclear receptor co-regulator; DNA repair, damage. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: cell surface; condensed chromosome; cytosol; early endosome; ER-Golgi intermediate compartment; extracellular region; extracellular space; nucleoplasm; nucleus; plasma membrane; transcriptional repressor complex. Molecular Function: bubble DNA binding; C-X-C chemokine binding; chemoattractant activity; cytokine activity; damaged DNA binding; DNA bending activity; double-stranded DNA binding; double-stranded RNA binding; four-way junction DNA binding; lipopolysaccharide binding; lyase activity; phosphatidylserine binding; protein binding; RAGE receptor binding; single-stranded DNA binding; single-stranded RNA binding; transcription factor activity; transcription factor binding. Biological Process: activation of innate immune response; apoptosis; apoptotic cell clearance; autophagy; base-excision repair; cell structure disassembly during apoptosis; chromatin assembly; dendritic cell chemotaxis; DNA fragmentation during apoptosis; DNA geometric change; DNA ligation during DNA repair; DNA recombination; DNA topological change; elevation of cytosolic calcium ion concentration; endothelial cell proliferation; inflammatory response; inflammatory response to antigenic stimulus; innate immune response; macrophage activation during immune response; myeloid dendritic cell activation; negative regulation of blood vessel endothelial cell migration; negative regulation of CD4-positive, alpha beta T cell differentiation; negative regulation of interferon-gamma production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; neurite development; plasmacytoid dendritic cell activation; positive chemotaxis; positive regulation of activated T cell proliferation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of DNA binding; positive regulation of DNA ligation; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-1 secretion; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of mismatch repair; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; programmed cell death; regulation of autophagy; regulation of restriction endodeoxyribonuclease activity; regulation of T cell mediated immune response to tumor cell; regulation of tolerance induction; regulation of transcription from RNA polymerase II promoter; T-helper 1 cell differentiation; V(D)J recombination

48-Strip-Wells

295 USD

96-Strip-Wells

390