Rat Interferon Beta ELISA Kit | MyBioSource MBS762629 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Interferon Beta ELISA Kit

catalog :

MBS762629

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS762629

products type :

ELISA Kit

products full name :

Rat Interferon Beta ELISA Kit

products short name :

[Interferon Beta]

products name syn :

[IFN-b/IFNB1/Fibroblast interferon/IFBIFNB/IFF/IFN-beta/interferon beta ; Interferon Beta]

other names :

[interferon beta; Interferon beta; interferon beta; interferon, beta 1, fibroblast; Fibroblast interferon]

products gene name :

[IFN-beta]

products gene name syn :

[IFN-b]

other gene names :

[IFNB1; IFNB1; IFB; IFF; IFNB; IFN-beta; IFB; IFNB; IFN-beta]

uniprot entry name :

IFNB_HUMAN

reactivity :

Rat

sequence length :

187

specificity :

This assay has high sensitivity and excellent specificity for detection of IFN-beta. No significant cross-reactivity or interference between IFN-beta and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/ml. Sensitivity: <9.375pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFN-beta were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFN-beta were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IFN-beta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IFN-beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IFN-beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IFN-beta can be calculated.

ncbi gi num :

4504603

ncbi acc num :

NP_002167.1

ncbi gb acc num :

NM_002176.3

ncbi mol weight :

22,294 Da

ncbi pathways :

Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (1269310); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833)

ncbi summary :

This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection. [provided by RefSeq, Sep 2015]

uniprot summary :

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: extracellular region; extracellular space. Molecular Function: chloramphenicol O-acetyltransferase activity; cytokine activity; interferon-alpha/beta receptor binding. Biological Process: adaptive immune response; B cell activation during immune response; B cell differentiation; B cell proliferation; blood coagulation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; defense response to bacterium; defense response to virus; humoral immune response; innate immune response; natural killer cell activation; natural killer cell activation during immune response; negative regulation of T cell differentiation; negative regulation of viral genome replication; positive regulation of innate immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of transcription from RNA polymerase II promoter; regulation of MHC class I biosynthetic process; response to exogenous dsRNA; response to virus; T cell activation during immune response

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425