ELISA/assay

Human Axin-1 ELISA Kit

MBS762601

48-Strip-Wells

300 USD

ELISA Kit

Human Axin-1 ELISA Kit

[Axin-1]

[AXIN1/axin 1/axin-1/AXINaxis inhibitor 1/Axis inhibition protein 1/fused; mouse; homolog of/hAxin/MGC52315]

[axin-1 isoform b; Axin-1; axin-1; axin 1; Axis inhibition protein 1; hAxin]

[AXIN1]

[AXIN1; AXIN1; AXIN; PPP1R49; AXIN; hAxin]

AXIN1_HUMAN

Human

826

This assay has high sensitivity and excellent specificity for detection of ATP13A2. No significant cross-reactivity or interference between ATP13A2 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP13A2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP13A2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ATP13A2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ATP13A2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ATP13A2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ATP13A2 can be calculated.

31083144

NP_851393.1

NM_181050.2

91,653 Da

AMER1 Mutants Destabilize The Destruction Complex Pathway (1268913); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1268909); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1268912); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1268910); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Beta-catenin Phosphorylation Cascade Pathway (1269597); C-MYC Pathway (169344); Canonical Wnt Signaling Pathway (138032); Colorectal Cancer Pathway (83106)

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. Protein type: Tumor suppressor; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: beta-catenin destruction complex; cell cortex; cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasmic microtubule; cytoplasmic vesicle; cytosol; lateral plasma membrane; nucleus; perinuclear region of cytoplasm; postsynaptic density. Molecular Function: beta-catenin binding; enzyme binding; GTPase activator activity; identical protein binding; p53 binding; protein binding; protein C-terminus binding; protein complex scaffold; protein homodimerization activity; protein kinase binding; protein self-association; receptor signaling complex scaffold activity; signal transducer activity; SMAD binding; ubiquitin protein ligase binding. Biological Process: activation of JNK activity; activation of protein kinase activity; apoptosis; axial mesoderm formation; cell death; cellular protein complex assembly; cytoplasmic microtubule organization and biogenesis; determination of left/right symmetry; dorsal/ventral axis specification; embryonic eye morphogenesis; forebrain anterior/posterior pattern formation; in utero embryonic development; muscle cell development; negative regulation of fat cell differentiation; negative regulation of protein metabolic process; negative regulation of Wnt receptor signaling pathway; nucleocytoplasmic transport; olfactory placode formation; optic placode formation; positive regulation of GTPase activity; positive regulation of JNK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of protein catabolic process; positive regulation of protein ubiquitination; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of ubiquitin-protein ligase activity; protein catabolic process; protein homooligomerization; protein polyubiquitination; sensory perception of sound; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; Wnt receptor signaling pathway through beta-catenin. Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425