Human Carboxypeptidase B2 ELISA Kit | MyBioSource MBS762600 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Carboxypeptidase B2 ELISA Kit

catalog :

MBS762600

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS762600

products type :

ELISA Kit

products full name :

Human Carboxypeptidase B2 ELISA Kit

products short name :

[Carboxypeptidase B2]

products name syn :

[CPB2 (Carboxypeptidase B2)/CPU/TAFI/carboxypeptidase B2/Carboxypeptidase U/PCPB/Plasma carboxypeptidase B/carboxypeptidase B-like protein/Thrombin-activable fibrinolysis inhibitor]

other names :

[carboxypeptidase B2 isoform 1 preproprotein; Carboxypeptidase B2; carboxypeptidase B2; carboxypeptidase B2; Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; TAFI]

products gene name :

[CPB2]

other gene names :

[CPB2; CPB2; CPU; PCPB; TAFI; CPU; pCPB; TAFI]

uniprot entry name :

CBPB2_HUMAN

reactivity :

Human

sequence length :

423

specificity :

This assay has high sensitivity and excellent specificity for detection of CPB2. No significant cross-reactivity or interference between CPB2 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 3.12-200ng/ml. Sensitivity: 1.875ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPB2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPB2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CPB2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CPB2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CPB2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CPB2 can be calculated.

ncbi gi num :

513126885

ncbi acc num :

NP_001863.3

ncbi gb acc num :

NM_001872.4

ncbi mol weight :

40,922 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Metabolism Of Angiotensinogen To Angiotensins Pathway (1268749); Metabolism Of Proteins Pathway (1268677); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Peptide Hormone Metabolism Pathway (1268746); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

uniprot summary :

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; EC 3.4.17.20; Secreted, signal peptide; Protease. Chromosomal Location of Human Ortholog: 13q14.11. Cellular Component: cell; extracellular space. Molecular Function: metallocarboxypeptidase activity; zinc ion binding. Biological Process: blood coagulation; fibrinolysis; negative regulation of fibrinolysis; proteolysis; response to drug; response to heat

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425