This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Matrix Metalloproteinase-9 ELISA Kit
catalog :
MBS762447
quantity :
48-Strip-Wells
price :
300 USD
product information
catalog number :
MBS762447
products type :
ELISA Kit
products full name :
Rat Matrix Metalloproteinase-9 ELISA Kit
products short name :
[Matrix Metalloproteinase-9]
products name syn :
[MMP-9 (Matrix Metalloproteinase 9)/CLG4B/Gelatinase B/GELB/macrophage gelatinase/MANDP2/MMP-9/type V collagenase]
other names :
[matrix metalloproteinase-9; matrix metalloproteinase-9]
products gene name :
[MMP-9]
other gene names :
[MMP9]
reactivity :
Rat
sequence length :
708
specificity :
This assay has high sensitivity and excellent specificity for detection of MMP-8. No significant cross-reactivity or interference between MMP-8 and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP-8 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP-8 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-8 can be calculated.
ncbi gi num :
83921637
ncbi acc num :
NP_001033093.1
ncbi gb acc num :
NM_001038004.1
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1337046); Axon Guidance Pathway (1337050); Bladder Cancer Pathway (84522); Bladder Cancer Pathway (527); Collagen Degradation Pathway (1337047); Degradation Of The Extracellular Matrix Pathway (1337045); Developmental Biology Pathway (1337049); EPH-Ephrin Signaling Pathway (1337070); EPH-ephrin Mediated Repulsion Of Cells Pathway (1337074); Estrogen Signaling Pathway (799191)
size1 :
48-Strip-Wells
price1 :
300 USD
size2 :
96-Strip-Wells
price2 :
415
size3 :
5x96-Strip-Wells
price3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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