ELISA/assay

Human Epidermal Growth Factor ELISA Kit

MBS762342

48-Strip-Wells

300 USD

ELISA Kit

Human Epidermal Growth Factor ELISA Kit

[Epidermal Growth Factor]

[EGF (Epidermal growth factor)/URG/HOMG4/Beta-Urogastrone]

[epidermal growth factor, partial; Pro-epidermal growth factor; pro-epidermal growth factor; epidermal growth factor; Epidermal growth factorAlternative name(s):Urogastrone]

[EGF]

[EGF; EGF; URG; HOMG4; EGF]

EGF_HUMAN

Human

53

This assay has high sensitivity and excellent specificity for detection of EEF1A1. No significant cross-reactivity or interference between EEF1A1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EEF1A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EEF1A1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-EEF1A1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EEF1A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EEF1A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EEF1A1 can be calculated.

374922206

AFA26280.1

129,746 Da

ARMS-mediated Activation Pathway (1269471); Adaptive Immune System Pathway (1269171); Arf6 Signaling Events Pathway (138034); Axon Guidance Pathway (1270303); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Ceramide Signaling Pathway (138023); Choline Metabolism In Cancer Pathway (1059539); Choline Metabolism In Cancer Pathway (1084232); Constitutive Signaling By Aberrant PI3K In Cancer Pathway (1268880)

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hormone; Ligand, receptor tyrosine kinase; Membrane protein, integral. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: extracellular region; extracellular space; lysosomal membrane; receptor complex. Molecular Function: epidermal growth factor receptor binding; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor protein tyrosine kinase activator activity; Wnt receptor activity; Wnt-protein binding. Biological Process: angiogenesis; DNA replication; epidermal growth factor receptor signaling pathway; MAPKKK cascade; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of secretion; phosphoinositide-mediated signaling; platelet degranulation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor activity; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of phosphorylation; positive regulation of receptor internalization; positive regulation of transcription, DNA-dependent; regulation of phosphoinositide 3-kinase cascade; signal transduction; STAT protein nuclear translocation; Wnt receptor signaling pathway through beta-catenin. Disease: Hypomagnesemia 4, Renal

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425