Mouse Gelsolin ELISA Kit | MyBioSource MBS762194 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Gelsolin ELISA Kit

catalog :

MBS762194

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS762194

products type :

ELISA Kit

products full name :

Mouse Gelsolin ELISA Kit

products short name :

[Gelsolin]

products name syn :

[GS (Gelsolin)/GSN/ADF/AGEL/Brevin/Actin-depolymerizing factor]

other names :

[gelsolin; Gelsolin; gelsolin; gelsolin; Actin-depolymerizing factor; ADF; Brevin]

products gene name :

[GS]

other gene names :

[Gsn; Gsn; ADF]

uniprot entry name :

GELS_RAT

reactivity :

Mouse

sequence length :

780

specificity :

This assay has high sensitivity and excellent specificity for detection of GS. No significant crossreactivity or interference between GS and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between GS and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.813-500ng/ml. Sensitivity: <4.688ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GS were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GS were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GS can be calculated.

ncbi gi num :

51854227

ncbi acc num :

NP_001004080.1

ncbi gb acc num :

NM_001004080.1

ncbi mol weight :

80,929 Da

ncbi pathways :

Apoptosis Pathway (1333181); Apoptotic Cleavage Of Cellular Proteins Pathway (1333206); Apoptotic Execution Phase Pathway (1333205); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1333207); Fc Gamma R-mediated Phagocytosis Pathway (114013); Fc Gamma R-mediated Phagocytosis Pathway (106590); Programmed Cell Death Pathway (1333180); Regulation Of Actin Cytoskeleton Pathway (198525); Regulation Of Actin Cytoskeleton Pathway (83481); Regulation Of Actin Cytoskeleton Pathway (500)

ncbi summary :

plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]

uniprot summary :

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation. Protein type: Motility/polarity/chemotaxis; Secreted; Actin-binding; Secreted, signal peptide; Nuclear receptor co-regulator. Cellular Component: actin cap; actin cytoskeleton; cortical actin cytoskeleton; cytoplasm; cytosol; extracellular region; extracellular space; focal adhesion; lamellipodium; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; protein complex; ruffle; sarcoplasm. Molecular Function: actin binding; calcium ion binding; myosin II binding; protein domain specific binding. Biological Process: actin filament capping; actin filament polymerization; actin filament severing; actin nucleation; actin polymerization and/or depolymerization; aging; apoptosis; barbed-end actin filament capping; extracellular matrix disassembly; negative regulation of virion penetration into host cell; oligodendrocyte development; phagocytosis, engulfment; phosphoinositide-mediated signaling; positive regulation of actin nucleation; protein destabilization; regulation of cell adhesion; response to ethanol; response to folic acid; sequestering of actin monomers; striated muscle atrophy; tissue regeneration; vesicle-mediated transport; wound healing

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage