ELISA/assay

Mouse Major urinary protein 20 ELISA Kit

MBS762149

48-Strip-Wells

300 USD

ELISA Kit

Mouse Major urinary protein 20 ELISA Kit

[Major urinary protein 20]

[MUP20/Darcin/Major urinary protein 24//Mup24]

[major urinary protein 20; Major urinary protein 20; major urinary protein 20; major urinary protein 20; Darcin]

[MUP20]

[Mup20; Mup20; Mup24; darcin; Gm12560; OTTMUSG00000007485]

MUP20_MOUSE

Mouse

181

This assay has high sensitivity and excellent specificity for detection of MUP20. No significant cross-reactivity or interference between MUP20 and analogues is observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Competitive. Detection Range: 0.312-20ng/ml. Sensitivity: <0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUP20 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUP20 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with MUP20. During the reaction, MUP20 in the sample or standard competes with a fixed amount of MUP20 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to MUP20. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MUP20 in the samples is then determined by comparing the O D of the samples to the standard curve.

59858561

NP_001012323.1

NM_001012323.1

20,930 Da

Mup20: Male pheromone which stimulates female sexual attraction to male urinary scent and promotes a strong learned attraction to the airborne urinary odor of an individual male. Promotes male agressive behavior. Binds most of the male pheromone, 2-sec-butyl- 4,5-dihydrothiazole, in urine. Belongs to the calycin superfamily. Lipocalin family. Cellular Component: extracellular region. Molecular Function: mating pheromone activity; pheromone activity; pheromone binding; transporter activity. Biological Process: olfactory learning; transport

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425