Hyaluronic Acid ELISA Kit | MyBioSource MBS761929 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Hyaluronic Acid ELISA Kit

catalog :

MBS761929

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS761929

products type :

ELISA Kit

products full name :

Hyaluronic Acid ELISA Kit

products short name :

[Hyaluronic Acid]

products name syn :

[HA (Hyaluronic Acid)]

other names :

[hyaluronic acid receptor; Lymphatic vessel endothelial hyaluronic acid receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; lymphatic vessel endothelial hyaluronan receptor 1; Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1; Hyaluronic acid receptor]

products gene name :

[HA]

other gene names :

[LYVE1; LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1; CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1]

uniprot entry name :

LYVE1_HUMAN

reactivity :

General

sequence length :

322

specificity :

This assay has high sensitivity and excellent specificity for detection of HA. No significant crossreactivity or interference between HA and analogues is observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 1.563-100ng/ml. Sensitivity: <0.938ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with HA. During the reaction, HA in the sample or standard competes with a fixed amount of HA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to HA. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HA in the samples is then determined by comparing the O D of the samples to the standard curve.

ncbi gi num :

10800122

ncbi acc num :

AAD49220.2

ncbi mol weight :

35,213 Da

ncbi pathways :

Glycosaminoglycan Metabolism Pathway (1269972); Hyaluronan Metabolism Pathway (1269973); Hyaluronan Uptake And Degradation Pathway (1269975); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957)

ncbi summary :

This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]

uniprot summary :

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 11p15. Cellular Component: integral to plasma membrane; membrane; plasma membrane. Molecular Function: hyaluronic acid binding; protein binding; receptor activity; transmembrane receptor activity. Biological Process: anatomical structure morphogenesis; carbohydrate metabolic process; cell motility; cell-matrix adhesion; glycosaminoglycan metabolic process; hyaluronan catabolic process; hyaluronan metabolic process; response to wounding; signal transduction; transport

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425