ELISA/assay

Rat Interleukin-2 Receptor-alpha ELISA Kit

MBS761893

48-Strip-Wells

300 USD

ELISA Kit

Rat Interleukin-2 Receptor-alpha ELISA Kit

[Interleukin-2 Receptor-alpha]

[IL-2Ralpha/CD25/Interleukin 2 Receptor Alpha; Interleukin-2 Receptor-a]

[interleukin-2 receptor alpha chain, partial; Interleukin-2 receptor subunit alpha; interleukin-2 receptor subunit alpha; interleukin 2 receptor subunit alpha; TAC antigen; p55; CD_antigen: CD25]

[IL-2Ralpha]

[IL-2Ra]

[IL2RA; IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA]

IL2RA_HUMAN

Rat

21

This assay has high sensitivity and excellent specificity for detection of IL-2R? . No significant cross-reactivity or interference between IL-2R? and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-2R ? were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL- 2R? were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-2R? antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-2R? antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-2R? amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-2R? can be calculated.

553510

AAA59162.1

30,819 Da

ARMS-mediated Activation Pathway (1269471); Arf6 Trafficking Events Pathway (137954); Axon Guidance Pathway (1270303); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Cytokine Signaling In Immune System Pathway (1269310); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284)

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 10p15-p14. Cellular Component: external side of plasma membrane; integral to membrane; intracellular; plasma membrane. Molecular Function: drug binding; interleukin-2 binding; interleukin-2 receptor activity. Biological Process: activated T cell apoptosis; activation of MAPKK activity; apoptosis; axon guidance; cell proliferation; cell surface receptor linked signal transduction; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; immune response; inflammatory response; inflammatory response to antigenic stimulus; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of defense response to virus; negative regulation of immune response; negative regulation of inflammatory response; negative regulation of T cell proliferation; nerve growth factor receptor signaling pathway; Notch signaling pathway; positive regulation of activated T cell proliferation; positive regulation of T cell differentiation; Ras protein signal transduction; regulation of T cell homeostatic proliferation; regulation of T cell tolerance induction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway. Disease: Diabetes Mellitus, Insulin-dependent, 10; Interleukin 2 Receptor, Alpha, Deficiency Of

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425