Bovine Haptoglobin ELISA Kit | MyBioSource MBS761858 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Haptoglobin ELISA Kit

catalog :

MBS761858

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS761858

products type :

ELISA Kit

products full name :

Bovine Haptoglobin ELISA Kit

products short name :

[Haptoglobin]

other names :

[haptoglobin; Haptoglobin; haptoglobin; haptoglobin; Zonulin]

products gene name :

[HP]

other gene names :

[HP; HP; BP; HPA1S; HP2ALPHA2]

uniprot entry name :

HPT_HUMAN

reactivity :

Bovine

sequence length :

406

specificity :

This assay has high sensitivity and excellent specificity for detection of HOPX. No significant cross-reactivity or interference between HOPX and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HOPX were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HOPX were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HOPX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HOPX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HOPX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HOPX can be calculated.

ncbi gi num :

386783

ncbi acc num :

AAA88080.1

ncbi mol weight :

38,452 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1269897); Scavenging Of Heme From Plasma Pathway (1269898); Vesicle-mediated Transport Pathway (1269876); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

uniprot summary :

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16q22.2. Cellular Component: extracellular region; extracellular space. Molecular Function: antioxidant activity; hemoglobin binding; protein binding; serine-type endopeptidase activity. Biological Process: acute-phase response; defense response; defense response to bacterium; immune system process; negative regulation of oxidoreductase activity; proteolysis; receptor-mediated endocytosis; response to hydrogen peroxide. Disease: Anhaptoglobinemia

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage