Human Placental Growth Factor ELISA Kit | MyBioSource MBS761718 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Placental Growth Factor ELISA Kit

catalog :

MBS761718

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS761718

products type :

ELISA Kit

products full name :

Human Placental Growth Factor ELISA Kit

products short name :

[Placental Growth Factor]

products name syn :

[PLGF/PGF (Placental Growth Factor)/Pgf/PGF/PGFL/placenta growth factor/placental growth factor/placental growth factor; vascular endothelial growth factor-related protein/PlGF/PlGF-2/PLGFplacental growth factor-like]

other names :

[Placental growth factor; Placenta growth factor; placenta growth factor; placental growth factor]

products gene name :

[PLGF]

other gene names :

[PGF; PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760; PGFL; PLGF; PlGF]

uniprot entry name :

PLGF_HUMAN

reactivity :

Human

sequence length :

170

specificity :

This assay has high sensitivity and excellent specificity for detection of Plet1. No significant cross-reactivity or interference between Plet1 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/ml. Sensitivity: <46.875pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plet1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plet1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Plet1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Plet1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Plet1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Plet1 can be calculated.

ncbi gi num :

14043631

ncbi acc num :

AAH07789.1

ncbi mol weight :

27,402 Da

ncbi pathways :

Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085); Signal Transduction Pathway (1269379)

ncbi summary :

This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

uniprot summary :

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q24.3. Cellular Component: extracellular region; extracellular space; membrane. Molecular Function: growth factor activity; heparin binding; protein binding; protein heterodimerization activity; protein homodimerization activity. Biological Process: cell differentiation; cell-cell signaling; cellular response to hormone stimulus; female pregnancy; organ regeneration; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; response to drug; response to hypoxia; signal transduction; sprouting angiogenesis; ureteric bud branching; vascular endothelial growth factor receptor signaling pathway

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage