Human Histone H4 ELISA Kit | MyBioSource MBS761642 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Histone H4 ELISA Kit

catalog :

MBS761642

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS761642

products type :

ELISA Kit

products full name :

Human Histone H4 ELISA Kit

products short name :

[Histone H4]

products name syn :

[HIST1H4A/Histone H4/H4/A/H4FA/H4F2/histone cluster 4; H4]

other names :

[histone H4; Histone H4; histone H4; histone cluster 4, H4]

products gene name :

[HIST1H4A]

other gene names :

[HIST4H4; HIST1H4A; H4/p; H4/A; H4FA]

uniprot entry name :

H4_HUMAN

reactivity :

Human

sequence length :

103

specificity :

This assay has high sensitivity and excellent specificity for detection of HIF-1alpha. No significant cross-reactivity or interference between HIF-1alpha and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 7.813-500pg/ml. Sensitivity: <4.688pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HIF-1alpha were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HIF-1alpha were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HIF-1alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HIF-1alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HIF-1alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HIF-1alpha can be calculated.

ncbi gi num :

28173560

ncbi acc num :

NP_778224.1

ncbi gb acc num :

NM_175054.2

ncbi mol weight :

11,367 Da

ncbi pathways :

Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway (1269513); Activation Of HOX Genes During Differentiation Pathway (1339139); Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway (1339140); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloid Fiber Formation Pathway (1269169); B-WICH Complex Positively Regulates RRNA Expression Pathway (1339126); Cell Cycle Pathway (1269741); Cell Cycle Checkpoints Pathway (1269742); Cell Cycle, Mitotic Pathway (1269763)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aug 2015]

uniprot summary :

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage