catalog number :
MBS761600
products type :
ELISA Kit
products full name :
Human Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA Kit
products short name :
[Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN]
products name syn :
[PTEN/Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN/Mutated in multiple advanced cancers 1/Phosphatase and tensin homolog/MMAC1/TEP1]
other names :
[phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN]
products gene name :
[PTEN]
other gene names :
[PTEN]
specificity :
This assay has high sensitivity and excellent specificity for detection of PSPC1. No significant cross-reactivity or interference between PSPC1 and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PSPC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PSPC1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PSPC1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PSPC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PSPC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PSPC1 can be calculated.
ncbi acc num :
NP_001306827.1
ncbi gb acc num :
NM_001319898.1
ncbi pathways :
Adaptive Immune System Pathway (1332122); Androgen Receptor Signaling Pathway (198553); Central Carbon Metabolism In Cancer Pathway (1059772); Central Carbon Metabolism In Cancer Pathway (1084231); DAP12 Interactions Pathway (1332219); DAP12 Signaling Pathway (1332220); Downstream TCR Signaling Pathway (1332127); Downstream Signal Transduction Pathway (1331458); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1332136); Downstream Signaling Of Activated FGFR1 Pathway (1331373)
size4 :
10x96-Strip-Wells