catalog number :
MBS761515
products type :
ELISA Kit
products full name :
Human Anti-Teichoic Acid Antibody ELISA Kit
products short name :
[Anti-Teichoic Acid Antibody]
products name syn :
[TA/Anti-Teichoic Acid Antibody]
products gene name :
[TA]
specificity :
This assay has high sensitivity and excellent specificity for detection of TA. No significant crossreactivity or interference between TA and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antigen. Detection Range: 4.688-300ng/ml. Sensitivity: 2.813ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on indirect enzyme-linked immune-sorbent assay technology. Teichoic Acid was pre-coated onto 96-well plates. And the Biotin- labeled Antibody was used as detection antibody. The standards, test samples and Biotin- labeled Antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TA amount of sample captured in plate. Read the OD absorbance at 450nm in a microplate reader, and then the concentration of TA can be calculated.
size4 :
10x96-Strip-Wells