Mouse Growth Differentiation Factor 10 ELISA Kit | MyBioSource MBS761487 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Growth Differentiation Factor 10 ELISA Kit

catalog :

MBS761487

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS761487

products type :

ELISA Kit

products full name :

Mouse Growth Differentiation Factor 10 ELISA Kit

products short name :

[Growth Differentiation Factor 10]

products name syn :

[BMP-3b/GDF10 (Bone morphogenetic protein 3B)/GDF-10/BIP/BMP3B/bone morphogenetic protein 3B/Bone-inducing protein/growth differentiation factor 10]

other names :

[growth differentiation factor 10; Growth/differentiation factor 10; growth/differentiation factor 10; growth differentiation factor 10; Bone morphogenetic protein 3B; BMP-3B; Bone-inducing protein; BIP]

products gene name :

[GDF10]

other gene names :

[GDF10; GDF10; BMP3B; BMP-3b; GDF-10; BMP-3B; BIP]

uniprot entry name :

GDF10_HUMAN

reactivity :

Mouse

sequence length :

478

specificity :

This assay has high sensitivity and excellent specificity for detection of GDF10. No significant cross-reactivity or interference between GDF10 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 0.156-10ng/ml . Sensitivity: 0.094ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDF10 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDF10 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GDF10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GDF10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDF10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDF10 can be calculated.

ncbi gi num :

119571046

ncbi acc num :

EAW50661.1

ncbi mol weight :

53,122 Da

ncbi pathways :

Adipogenesis Pathway (198832)

ncbi summary :

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

uniprot summary :

GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 10q11.22. Cellular Component: extracellular space. Molecular Function: cytokine activity; growth factor activity; transforming growth factor beta receptor binding. Biological Process: BMP signaling pathway; cell development; fat cell differentiation; negative regulation of osteoblast differentiation; osteoblast differentiation; regulation of apoptosis; regulation of MAPKKK cascade; skeletal development; transforming growth factor beta receptor signaling pathway

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage