ELISA/assay

Mouse von Willebrand factor ELISA Kit

MBS761484

48-Strip-Wells

300 USD

ELISA Kit

Mouse von Willebrand factor ELISA Kit

[von Willebrand factor]

[Vwf/F8VWF/VWF/F8VWF/VWD/VWF von Willebrand factor]

[von Willebrand factor preproprotein; von Willebrand factor; von Willebrand factor; von Willebrand factor; von Willebrand antigen 2Alternative name(s):von Willebrand antigen II]

[Vwf]

[VWF; VWF; VWD; F8VWF; F8VWF; vWF]

VWF_HUMAN

Mouse

2813

This assay has high sensitivity and excellent specificity for detection of Vwf. No significant cross-reactivity or interference between Vwf and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between Vwf and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.25-80ng/ml. Sensitivity: <0.75ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vwf were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vwf were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Vwf antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Vwf antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Vwf amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Vwf can be calculated.

89191868

NP_000543.2

NM_000552.3

38,745 Da

ARMS-mediated Activation Pathway (1269471); Axon Guidance Pathway (1270303); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Developmental Biology Pathway (1270302)

This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015]

VWF: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Defects in VWF are the cause of von Willebrand disease type 1 (VWD1). A common hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 1 is characterized by partial quantitative deficiency of circulating von Willebrand factor, that is otherwise structurally and functionally normal. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma. Defects in VWF are the cause of von Willebrand disease type 2 (VWD2). A hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 2 is characterized by qualitative deficiency and functional anomalies of von Willebrand factor. It is divided in different subtypes including 2A, 2B, 2M and 2N (Normandy variant). The mutant VWF protein in types 2A, 2B and 2M are defective in their platelet- dependent function, whereas the mutant protein in type 2N is defective in its ability to bind factor VIII. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma. Defects in VWF are the cause of von Willebrand disease type 3 (VWD3). A severe hemorrhagic disorder due to a total or near total absence of von Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. Bleeding usually starts in infancy and can include epistaxis, recurrent mucocutaneous bleeding, excessive bleeding after minor trauma, and hemarthroses. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Cell adhesion; Extracellular matrix. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: endoplasmic reticulum; external side of plasma membrane; extracellular matrix; extracellular region; proteinaceous extracellular matrix. Molecular Function: chaperone binding; collagen binding; glycoprotein binding; identical protein binding; immunoglobulin binding; integrin binding; protease binding; protein binding; protein homodimerization activity; protein N-terminus binding. Biological Process: blood coagulation; blood coagulation, intrinsic pathway; cell adhesion; cell-substrate adhesion; extracellular matrix organization and biogenesis; hemostasis; liver development; placenta development; platelet activation; platelet degranulation; protein homooligomerization; response to wounding. Disease: Von Willebrand Disease, Type 1; Von Willebrand Disease, Type 2; Von Willebrand Disease, Type 3

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425