ELISA/assay

Mouse Serine protease inhibitor A3N ELISA Kit

MBS761385

48-Strip-Wells

300 USD

ELISA Kit

Mouse Serine protease inhibitor A3N ELISA Kit

[Serine protease inhibitor A3N]

[Serpina3n/Spi2/Serine protease inhibitor A3N/Serpin A3N]

[serine protease inhibitor A3N; Serine protease inhibitor A3N; serine protease inhibitor A3N; serine (or cysteine) peptidase inhibitor, clade A, member 3N; CPI-26; Contrapsin-like protease inhibitor 6; SPI-2.2; Serine protease inhibitor 3; SPI-3]

[Serpina3n]

[Serpina3n; Serpina3n; SPI3; Spin3; CPi-26; Spin2c; Spi-2.2; Spin2c; Serpin A3N; SPI-3]

SPA3N_RAT

Mouse

408

This assay has high sensitivity and excellent specificity for detection of SEPP1. No significant cross-reactivity or interference between SEPP1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/ml. Sensitivity: <0.469ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEPP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEPP1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SEPP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SEPP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SEPP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SEPP1 can be calculated.

13928716

NP_113719.1

NM_031531.1

46,652 Da

secreted acidic glycoprotein that may act as a serine protease inhibitor; expression is transiently induced during inflammation [RGD, Feb 2006]

SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region; extracellular space. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: inflammatory response; response to cytokine stimulus; response to lipopolysaccharide; response to peptide hormone stimulus

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425