ELISA/assay

Rat Leptin ELISA Kit

MBS761173

48-Strip-Wells

300 USD

ELISA Kit

Rat Leptin ELISA Kit

[Leptin]

[LEP (Leptin)/LEPD/OBS/Obesity Homolog]

[leptin; Leptin; leptin; leptin; Obese protein; Obesity factor]

[Lep]

[LEP; LEP; OB; OBS; LEPD]

LEP_HUMAN

Rat

167

This assay has high sensitivity and excellent specificity for detection of LELP1. No significant cross-reactivity or interference between LELP1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 78-5000pg/ml. Sensitivity: <46.9pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LELP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LELP1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-LELP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-LELP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LELP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LELP1 can be calculated.

4557715

NP_000221.1

NM_000230.2

18,641 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1270302); HIF-1-alpha Transcription Factor Network Pathway (138045)

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family. Protein type: Hormone; Secreted; Cell development/differentiation; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q31.3. Cellular Component: cytoplasm; extracellular region; extracellular space. Molecular Function: growth factor activity; hormone activity; peptide hormone receptor binding. Biological Process: adult feeding behavior; bile acid metabolic process; central nervous system neuron development; cholesterol metabolic process; circadian rhythm; eating behavior; energy reserve metabolic process; fatty acid beta-oxidation; female pregnancy; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process; hormone metabolic process; insulin secretion; intestinal absorption; leptin-mediated signaling pathway; leukocyte tethering or rolling; negative regulation of apoptosis; negative regulation of appetite; negative regulation of autophagy; negative regulation of glucose import; negative regulation of transcription from RNA polymerase II promoter; negative regulation of vasoconstriction; ovulation from ovarian follicle; placenta development; positive regulation of cytokine production; positive regulation of developmental growth; positive regulation of follicle-stimulating hormone secretion; positive regulation of insulin receptor signaling pathway; positive regulation of ion transport; positive regulation of JAK-STAT cascade; positive regulation of luteinizing hormone secretion; positive regulation of MAPKKK cascade; positive regulation of myeloid cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of TOR signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; prostaglandin secretion; regulation of angiogenesis; regulation of blood pressure; regulation of bone remodeling; regulation of cell cycle; regulation of cholesterol absorption; regulation of endothelial cell proliferation; regulation of gluconeogenesis; regulation of insulin secretion; regulation of natural killer cell activation; regulation of natural killer cell mediated cytotoxicity; regulation of natural killer cell proliferation; regulation of nitric-oxide synthase activity; regulation of steroid biosynthetic process; response to activity; response to dietary excess; response to estradiol stimulus; response to ethanol; response to hypoxia; response to insulin stimulus; response to vitamin E; sexual reproduction; T cell differentiation; tyrosine phosphorylation of STAT protein. Disease: Leptin Deficiency

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425