catalog number :
MBS761112
products type :
ELISA Kit
products full name :
Rat Dehydroepiandrosterone ELISA Kit
products short name :
[Dehydroepiandrosterone]
products name syn :
[DHEA]
products gene name :
[DHEA]
specificity :
This assay has high sensitivity and excellent specificity for detection of DHEA. No significant cross-reactivity or interference between DHEA and analogues is observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Standard Curve/Testing Data
other info1 :
Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Competitive. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DHEA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DHEA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with DHEA. During the reaction, DHEA in the sample or standard competes with a fixed amount of DHEA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to DHEA. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DHEA in the samples is then determined by comparing the O D of the samples to the standard curve.
size4 :
10x96-Strip-Wells