ELISA/assay

Human Integrin beta-1 ELISA Kit

MBS761086

48-Strip-Wells

300 USD

ELISA Kit

Human Integrin beta-1 ELISA Kit

[Integrin beta-1]

[ITGB1/CD29/CD29 antigen/Fibronectin receptor subunit beta/FNRBVLAB/GPIIA/integrin beta-1/integrin VLA-4 beta subunit/integrin; beta 1 (fibronectin receptor; beta polypeptide; antigen CD29 includesMDF2; MSK12)/MDF2/MSK12/very late activation protein; beta polypeptide/VLA-4 subunit beta/VLA-BETA]

[integrin beta-1 isoform 1D; Integrin beta-1; integrin beta-1; integrin subunit beta 1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD_antigen: CD29]

[ITGB1]

[ITGB1; ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA; FNRB; MDF2; MSK12; GPIIA]

ITB1_HUMAN

Human

801

This assay has high sensitivity and excellent specificity for detection of ITGA4. No significant cross-reactivity or interference between ITGA4 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: < 0.094ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGA4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGA4were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ITGA4antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ITGA4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ITGA4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ITGA4 can be calculated.

19743819

NP_391988.1

NM_033668.2

88,884 Da

Adaptive Immune System Pathway (1269171); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (1270303); Bacterial Invasion Of Epithelial Cells Pathway (149807)

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle). Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.; Cell surface. Chromosomal Location of Human Ortholog: 10p11.2. Cellular Component: acrosome; basement membrane; cell surface; cleavage furrow; cytoplasm; dendritic spine; external side of plasma membrane; filopodium; focal adhesion; hemidesmosome; integrin complex; lamellipodium; lipid raft; melanosome; membrane; neuromuscular junction; perinuclear region of cytoplasm; plasma membrane; receptor complex; recycling endosome; ruffle; sarcolemma. Molecular Function: actin binding; alpha-actinin binding; cell adhesion molecule binding; fibronectin binding; glycoprotein binding; integrin binding; laminin binding; metal ion binding; peptide binding; protease binding; protein binding; protein complex binding; protein domain specific binding; protein heterodimerization activity; protein kinase binding; viral receptor activity. Biological Process: axon extension; axon guidance; B cell differentiation; blood coagulation; calcium-independent cell-matrix adhesion; cardiac muscle cell differentiation; cell fate specification; cell migration; cell migration during sprouting angiogenesis; cell-cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; cellular calcium ion homeostasis; cellular defense response; dendrite morphogenesis; entry of virus into host cell; extracellular matrix organization and biogenesis; formation of radial glial scaffolds; G1/S transition of mitotic cell cycle; germ cell migration; heterotypic cell-cell adhesion; homophilic cell adhesion; in utero embryonic development; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; maternal process involved in pregnancy; mesodermal cell differentiation; negative regulation of cell projection organization and biogenesis; negative regulation of cell proliferation; negative regulation of neuron differentiation; negative regulation of Rho protein signal transduction; positive regulation of apoptosis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endocytosis; positive regulation of GTPase activity; positive regulation of MAPKKK cascade; positive regulation of peptidyl-tyrosine phosphorylation; protein transport within lipid bilayer; receptor internalization; regulation of cell cycle; regulation of G-protein coupled receptor protein signaling pathway; regulation of immune response; response to activity; response to drug; sarcomere organization; small GTPase mediated signal transduction; stress fiber formation; tissue homeostasis; transforming growth factor beta receptor signaling pathway; visual learning

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425