catalog number :
MBS760745
products type :
ELISA Kit
products full name :
Mouse Intestinal Fatty Acid Binding Protein ELISA Kit
products short name :
[Intestinal Fatty Acid Binding Protein]
products name syn :
[IFABP/FABP2/FABPI/I-FABP/I-FABP/Intestinal FABP/FABPI/fatty acid binding protein 2; intestinal/Fatty acid-binding protein 2/fatty acid-binding protein; intestinal/I-FABP/Intestinal-type fatty acid-binding protein]
other names :
[intestinal fatty acid binding protein; Intestinal fatty acid binding protein; fatty acid-binding protein, intestinal; Intestinal fatty acid binding protein]
products gene name :
[IFABP/FABP2]
other gene names :
[FABP2; I-FABP; I-FABP]
uniprot entry name :
F8UN39_COLLI
specificity :
This assay has high sensitivity and excellent specificity for detection of IDO1. No significant cross-reactivity or interference between IDO1 and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IDO1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IDO1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IDO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IDO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IDO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IDO1 can be calculated.
ncbi acc num :
AEH05982.1
ncbi mol weight :
15,134 Da
ncbi pathways :
PPAR Signaling Pathway (916955); PPAR Signaling Pathway (450)
size4 :
10x96-Strip-Wells