Human Selenoprotein P ELISA Kit | MyBioSource MBS760712 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Selenoprotein P ELISA Kit

catalog :

MBS760712

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS760712

products type :

ELISA Kit

products full name :

Human Selenoprotein P ELISA Kit

products short name :

[Selenoprotein P]

products name syn :

[SEPP1 (Selenoprotein P)/SELP/SeP]

other names :

[selenoprotein P; Selenoprotein P; selenoprotein P; selenoprotein P, plasma, 1]

products gene name :

[SEPP1]

other gene names :

[SEPP1; SEPP1; SeP; SELP; SEPP; SELP; SeP]

uniprot entry name :

SEPP1_HUMAN

reactivity :

Human

sequence length :

381

specificity :

This assay has high sensitivity and excellent specificity for detection of sEPCR. No significant cross-reactivity or interference between sEPCR and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level sEPCR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level sEPCR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-sEPCR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-sEPCR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the sEPCR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of sEPCR can be calculated.

ncbi gi num :

312004116

ncbi acc num :

CAA77836.2

uniprot acc num :

P49908

ncbi mol weight :

43,174 Da

ncbi pathways :

Integrin-mediated Cell Adhesion Pathway (198816); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825)

ncbi summary :

This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q31. Cellular Component: extracellular region; extracellular space. Molecular Function: selenium binding. Biological Process: brain development; growth; locomotory behavior; post-embryonic development; response to oxidative stress; selenium metabolic process; sexual reproduction

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage