ELISA/assay

Rat IL-22 (Interleukin 22) ELISA Kit

MBS7606913

48-Strip-Wells

300 USD

ELISA Kit

Rat IL-22 (Interleukin 22) ELISA Kit

[IL-22 (Interleukin 22)]

[interleukin 22; Interleukin-22; interleukin-22; interleukin 22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-TIF]

[IL-22]

[IL22; IL22; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23; ILTIF; ZCYTO18; IL-22; IL-TIF]

Rat

179

This assay has high sensitivity and excellent specificity for detection ofIL-1beta. No significant cross-reactivity or interference betweenIL-1betaand analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenatesand Other Biological Fluids. Assay Type: Sandwich. Detection Range: 3.906-250pg/ml. Sensitivity: <2.344pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelIL-1betawere tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelIL-1betawere tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1betaantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1beta can be calculated.

10719562

AAG22064.1

20,011 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Inflammatory Bowel Disease Pathway (842771); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family. Protein type: Cytokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q15. Cellular Component: extracellular region. Molecular Function: protein binding. Biological Process: cell-cell signaling

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425