Mouse IL-8 (Interleukin 8) ELISA Kit | MyBioSource MBS7606860 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse IL-8 (Interleukin 8) ELISA Kit

catalog :

MBS7606860

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS7606860

products type :

ELISA Kit

products full name :

Mouse IL-8 (Interleukin 8) ELISA Kit

products short name :

[IL-8 (Interleukin 8)]

other names :

[interleukin-8; Interleukin-8; interleukin-8; C-X-C motif chemokine ligand 8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1]

products gene name :

[IL-8]

other gene names :

[CXCL8; CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1; IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF]

reactivity :

Mouse

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of IL-8. No significant cross-reactivity or interference between IL-8 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/ml. Sensitivity: <9.375pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-8 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-8 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-8 can be calculated.

ncbi gi num :

10834978

ncbi acc num :

NP_000575.1

ncbi gb acc num :

NM_000584.3

uniprot acc num :

P10145

ncbi mol weight :

11,098 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Activation Of Genes By ATF4 Pathway (530772); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359)

ncbi summary :

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]

uniprot summary :

IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular region; extracellular space. Molecular Function: chemokine activity; interleukin-8 receptor binding; protein binding. Biological Process: angiogenesis; calcium-mediated signaling; cell cycle arrest; cell motility; chemotaxis; embryonic gut development; G-protein coupled receptor protein signaling pathway; immune response; induction of positive chemotaxis; inflammatory response; negative regulation of cell proliferation; negative regulation of G-protein coupled receptor protein signaling pathway; neutrophil activation; neutrophil chemotaxis; positive regulation of angiogenesis; receptor internalization; regulation of cell adhesion; regulation of retroviral genome replication; response to molecule of bacterial origin; signal transduction

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425