ELISA/assay

Bovine IL-10 (Interleukin 10) ELISA Kit

MBS7606831

48-Strip-Wells

300 USD

ELISA Kit

Bovine IL-10 (Interleukin 10) ELISA Kit

[IL-10 (Interleukin 10)]

[Interleukin-10; Interleukin-10; interleukin-10; Cytokine synthesis inhibitory factor; CSIF]

[IL-10]

[IL10; IL10; IL-10; CSIF]

Bovine

178

This assay has high sensitivity and excellent specificity for detection of IL-10. No significant cross-reactivity or interference between IL-10 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 6.25-400pg/ml. Sensitivity: <3.75pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-10 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-10 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-10 can be calculated.

172046854

P43480.2

P43480

20,438 Da

African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (1458350); Allograft Rejection Pathway (84358); Allograft Rejection Pathway (535); Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Asthma Pathway (84355); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84356)

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425