product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse 8-iso-PGF2alpha (8-isoprostane) ELISA Kit
catalog :
MBS7606827
quantity :
48-Strip-Wells
price :
300 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS7606827
products type :
ELISA Kit
products full name :
Mouse 8-iso-PGF2alpha (8-isoprostane) ELISA Kit
products short name :
[8-iso-PGF2alpha (8-isoprostane)]
products gene name :
[8-iso-PGF2alpha]
reactivity :
Mouse
specificity :
This assay has high sensitivity and excellent specificity for detection of 6-hydroxymelatonin sulfate. No significant cross-reactivity or interference between 6-hydroxymelatonin sulfate and analogues is observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 7.813-500pg/ml. Sensitivity: <4.688pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 6-hydroxymelatonin sulfate were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 6-hydroxymelatonin sulfate were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with 6-hydroxymelatonin sulfate. During the reaction, 6-hydroxymelatonin sulfate in the sample or standard competes with a fixed amount of 6-hydroxymelatonin sulfate on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 6-hydroxymelatonin sulfate. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 6-hydroxymelatonin sulfate in the samples is then determined by comparing the O D of the samples to the standard curve.
size1 :
48-Strip-Wells
price1 :
300 USD
size2 :
96-Strip-Wells
price2 :
415
size3 :
5x96-Strip-Wells
price3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
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