catalog number :
MBS7606803
products type :
ELISA Kit
products full name :
Human calprotectin ELISA Kit
products short name :
[calprotectin]
other names :
[calprotectin=urinary stone protein band A {N-terminal}]
specificity :
This assay has high sensitivity and excellent specificity for detection of calprotectin. No significant cross-reactivity or interference between calprotectin and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Mouse Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernatants. Assay Type: Sandwich. Detection Range: 31.2 pg/ml-2000 pg/ml. Sensitivity: < 2 pg/ml
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BDNF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BDNF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BDNF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BDNF can be calculated.
ncbi acc num :
AAB33355.1
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells