ELISA/assay

Bovine IL-1b (Interleukin 1 Beta) ELISA Kit

MBS7606685

48-Strip-Wells

300 USD

ELISA Kit

Bovine IL-1b (Interleukin 1 Beta) ELISA Kit

[IL-1b (Interleukin 1 Beta)]

[interleukin 1, beta; interleukin 1, beta]

[IL-1b]

[IL1B2; IL1B; IL-1beta]

Bovine

267

This assay has high sensitivity and excellent specificity for detection of IL-1beta. No significant cross-reactivity or interference between IL-1beta and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/ml. Sensitivity: <37.5pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-1 beta were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-1 beta were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1beta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1beta can be calculated.

697663067

NP_001289317.1

NM_001302388.1

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48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425