catalog number :
MBS7606640
products type :
ELISA Kit
products full name :
Mouse Calprotectin (Calprotectin) ELISA Kit
products short name :
[Calprotectin (Calprotectin)]
other names :
[calprotectin=urinary stone protein band A {N-terminal}]
specificity :
This assay has high sensitivity and excellent specificity for detection of Calprotectin. No significant cross-reactivity or interference between Calprotectin and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates and other biological fluids. Detection Range: 7.8-500ng/ml. Sensitivity: <4.688ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calprotectin were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calprotectin were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Calprotectin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Calprotectin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP- treptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Calprotectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Calprotectin can be calculated.
ncbi acc num :
AAB33355.1
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells