Mouse FUR (Furin) ELISA Kit | MyBioSource MBS7606595 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse FUR (Furin) ELISA Kit

catalog :

MBS7606595

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS7606595

products type :

ELISA Kit

products full name :

Mouse FUR (Furin) ELISA Kit

products short name :

[FUR (Furin)]

other names :

[Furin; Furin; furin; furin (paired basic amino acid cleaving enzyme); Dibasic-processing enzyme; Paired basic amino acid residue-cleaving enzyme; PACE; Prohormone convertase 3]

products gene name :

[FUR]

other gene names :

[Furin; Furin; Fur; PACE; SPC1; Pcsk3; 9130404I01Rik; Fur; Pcsk3; PACE]

reactivity :

Mouse

sequence length :

793

specificity :

This assay has high sensitivity and excellent specificity for detection of FUR . No significant cross-reactivity or interference between FUR and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between FUR and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 46.88-3000pg/ml. Sensitivity: <28.125pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FUR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FUR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FUR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FUR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FUR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FUR can be calculated.

ncbi gi num :

341940726

ncbi acc num :

P23188.2

uniprot acc num :

P23188

ncbi mol weight :

86,772 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1367347); Chylomicron-mediated Lipid Transport Pathway (1367662); Collagen Degradation Pathway (1367348); Degradation Of The Extracellular Matrix Pathway (1367346); Delta-Notch Signaling Pathway (198316); Developmental Biology Pathway (1366708); Elastic Fibre Formation Pathway (1367340); Extracellular Matrix Organization Pathway (1367333); Formation Of The Cornified Envelope Pathway (1457933); Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (1367228)

ncbi summary :

This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

uniprot summary :

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily. Protein type: Cell surface; EC 3.4.21.75; Membrane protein, integral; Protease; Vesicle. Chromosomal Location of Human Ortholog: 7 D2 7 45.65 cM. Cellular Component: cell surface; early endosome; endoplasmic reticulum; endoplasmic reticulum membrane; Golgi cisterna; Golgi membrane; integral to membrane; lipid raft; membrane; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle; trans-Golgi network transport vesicle membrane. Molecular Function: endopeptidase activity; nerve growth factor binding; peptidase activity; peptide binding; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity. Biological Process: cell proliferation; negative regulation of low-density lipoprotein receptor catabolic process; negative regulation of transforming growth factor-beta1 production; nerve growth factor production; peptide biosynthetic process; peptide hormone processing; positive regulation of cell migration; positive regulation of transforming growth factor beta receptor signaling pathway; protein processing; regulation of endopeptidase activity; regulation of low-density lipoprotein receptor biosynthetic process; regulation of protein catabolic process; regulation of signal transduction; secretion by cell; signal peptide processing; zymogen activation

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425