ELISA/assay

Human CORT (Corticosterone) ELISA Kit

MBS7606454

48-Strip-Wells

300 USD

ELISA Kit

Human CORT (Corticosterone) ELISA Kit

[CORT (Corticosterone)]

[Cortistatin; Cortistatin; cortistatin; cortistatin]

[CORT]

[CORT; CORT; CST-14; CST-17; CST-29]

Human

105

This assay has high sensitivity and excellent specificity for detection of CORT. No significant cross-reactivity or interference between CORT and analogues is observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 2.81-180ng/ml. Sensitivity: <1.688ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CORT were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CORT were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to CORT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CORT in the samples is then determined by comparing the O D of the samples to the standard curve.

3023540

O00230.1

O00230

11,532 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (i) Signalling Events Pathway (1269576); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543)

This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors, and shares many pharmacological and functional properties with somatostatin, including the depression of neuronal activity. However, it also has many properties distinct from somatostatin, such as induction of slow-wave sleep, apparently by antagonism of the excitatory effects of acetylcholine on the cortex, reduction of locomotor activity, and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing, TAF9-like domain 1) gene, as represented in GeneID:100526739. [provided by RefSeq, Nov 2010]

CORT: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs. Belongs to the somatostatin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.22. Cellular Component: extracellular region. Molecular Function: chromatin binding; double-stranded DNA binding; G-protein-coupled receptor binding; neuropeptide hormone activity. Biological Process: G-protein signaling, adenylate cyclase inhibiting pathway; mitotic recombination; replication fork processing; resolution of meiotic joint molecules as recombinants

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425