catalog number :
MBS7606430
products type :
ELISA Kit
products full name :
Porcine MAP (major acute phase protein) ELISA Kit
products short name :
[MAP (major acute phase protein)]
products name syn :
[Inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; Inter-alpha-inhibitor heavy chain 4; ITIH4; IHRP]
products gene name :
[MAP]
specificity :
This assay has high sensitivity and excellent specificity for detection of MAP. No significant cross-reactivity or interference between MAP and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.563-100ng/ml. Sensitivity: <0.938ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MAP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MAP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MAP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MAP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAP can be calculated.
size4 :
10x96-Strip-Wells