Mouse LBP (Lipolysaccharide binding protein) ELISA Kit | MyBioSource MBS7606429 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse LBP (Lipolysaccharide binding protein) ELISA Kit

catalog :

MBS7606429

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7606429

products type :

ELISA Kit

products full name :

Mouse LBP (Lipolysaccharide binding protein) ELISA Kit

products short name :

[LBP (Lipolysaccharide binding protein)]

other names :

[Lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; lipopolysaccharide binding protein]

products gene name :

[LBP]

other gene names :

[Lbp; Lbp; Ly88; Bpifd2; LBP]

reactivity :

Mouse

sequence length :

481

specificity :

This assay has high sensitivity and excellent specificity for detection of LAPTM4B. No significant cross-reactivity or interference between LAPTM4B and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.469-30ng/ml. Sensitivity: <0.281ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LAPTM4B were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LAPTM4B were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-LAPTM4B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-LAPTM4B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LAPTM4B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LAPTM4B can be calculated.

ncbi gi num :

341940900

ncbi acc num :

Q61805.2

uniprot acc num :

Q61805

ncbi mol weight :

53,055 Da

ncbi pathways :

Immune System Pathway (971216); Innate Immune System Pathway (971212); NF-kappa B Signaling Pathway (634537); Salmonella Infection Pathway (375174); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (970702); Toll-Like Receptors Cascades Pathway (1001207); Toll-like Receptor Signaling Pathway (83273); Toll-like Receptor Signaling Pathway (487); Transfer Of LPS From LBP Carrier To CD14 Pathway (1001233)

uniprot summary :

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2 H1 2 78.72 cM. Cellular Component: cell surface; extracellular space. Molecular Function: lipopolysaccharide binding; receptor binding. Biological Process: acute-phase response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; detection of molecule of bacterial origin; innate immune response; leukocyte chemotaxis during inflammatory response; lipopolysaccharide transport; lipopolysaccharide-mediated signaling pathway; macromolecule localization; macrophage activation during immune response; negative regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of cytolysis; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of macrophage activation; positive regulation of phagocytosis, engulfment; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; response to lipopolysaccharide

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage