catalog number :
MBS7606315
products type :
ELISA Kit
products full name :
Mouse ANA (Anti-nuclear Antibody) ELISA Kit
products short name :
[ANA (Anti-nuclear)]
products name syn :
[Anti-nuclear Antibody]
products gene name :
[ANA]
specificity :
This assay has high sensitivity and excellent specificity for detection of ACCPA. No significant cross-reactivity or interference between ACCPA and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/ml. Sensitivity: <0.938ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ACCPA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ACCPA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. CCP was precoated onto 96-well plates. And the biotin conjugated CCP was used as detection antigen. The standards, test samples and biotin conjugated detection Antigen were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ACCPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ACCPA can be calculated.
size4 :
10x96-Strip-Wells