Rat AKR1B1 (Aldose reductase) ELISA Kit | MyBioSource MBS7606282 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat AKR1B1 (Aldose reductase) ELISA Kit

catalog :

MBS7606282

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS7606282

products type :

ELISA Kit

products full name :

Rat AKR1B1 (Aldose reductase) ELISA Kit

products short name :

[AKR1B1 (Aldose reductase)]

other names :

[Aldose reductase; Aldose reductase; aldose reductase; aldo-keto reductase family 1 member B; Aldehyde reductase]

products gene name :

[AKR1B1]

other gene names :

[Akr1b1; Akr1b1; Alr; Aldr1; ALDRED; Akr1b3; Akr1b4; ALR-P-I; RATALDRED; Akr1b4; Aldr1; AR]

reactivity :

Rat

sequence length :

316

specificity :

This assay has high sensitivity and excellent specificity for detection of AKR1B1. No significant cross-reactivity or interference between AKR1B1 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AKR1B1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AKR1B1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AKR1B1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AKR1B1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AKR1B1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AKR1B1 can be calculated.

ncbi gi num :

1168407

ncbi acc num :

P07943.3

uniprot acc num :

P07943

ncbi mol weight :

35,797 Da

ncbi pathways :

Fructose Metabolism In Proximal Tubules Pathway (1458504); Fructose And Mannose Metabolism Pathway (83323); Fructose And Mannose Metabolism Pathway (291); Fructose Biosynthesis Pathway (1377560); Fructose Metabolism Pathway (1377559); Galactose Metabolism Pathway (83324); Galactose Metabolism Pathway (292); Glycerolipid Metabolism Pathway (83378); Glycerolipid Metabolism Pathway (361); Hexoses Metabolism In Proximal Tubules Pathway (1468872)

ncbi summary :

regulates NF-kappa B mediated mitogenic signaling; may play a role in the polyol pathway [RGD, Feb 2006]

uniprot summary :

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family. Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Oxidoreductase. Chromosomal Location of Human Ortholog: 4q22. Cellular Component: cytosol; extracellular space; mast cell granule; nucleoplasm; perinuclear region of cytoplasm. Molecular Function: aldehyde reductase activity; glyceraldehyde oxidoreductase activity. Biological Process: maternal process involved in pregnancy; monosaccharide metabolic process; naphthalene metabolic process; negative regulation of apoptosis; norepinephrine metabolic process; positive regulation of JAK-STAT cascade; positive regulation of smooth muscle cell proliferation; renal water homeostasis; response to organic substance; response to water deprivation; sorbitol biosynthetic process; stress-activated protein kinase signaling pathway; tissue homeostasis

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage