ELISA/assay

Human suPAR (soluble urokinase-type plasminogen activator receptor) ELISA Kit

MBS7606253

48-Strip-Wells

300 USD

ELISA Kit

Human suPAR (soluble urokinase-type plasminogen activator receptor) ELISA Kit

[suPAR (soluble urokinase-type plasminogen activator receptor)]

[soluble urokinase-type plasminogen activator receptor]

[soluble urokinase plasminogen activator receptor; Urokinase plasminogen activator surface receptor; urokinase plasminogen activator surface receptor; plasminogen activator, urokinase receptor; Monocyte activation antigen Mo3; CD_antigen: CD87]

[suPAR]

[PLAUR; PLAUR; CD87; UPAR; URKR; U-PAR; MO3; UPAR; U-PAR; uPAR]

Human

301

This assay has high sensitivity and excellent specificity for detection of suPAR. No significant cross-reactivity or interference between suPAR and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates and other biological fluids. Detection Range: 15.625-1000pg/ml. Sensitivity: <9.375pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level suPAR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level suPAR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- suPAR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- suPAR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the suPAR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of suPAR can be calculated.

13641309

AAK31795.1

32,016 Da

Arf6 Downstream Pathway (138038); Attachment Of GPI Anchor To UPAR Pathway (106239); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); FGF Signaling Pathway (137989); Hemostasis Pathway (106028); Metabolism Of Proteins Pathway (106230); Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway (106236)

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Receptor, misc. Chromosomal Location of Human Ortholog: 19q13.31. Cellular Component: cell surface; endoplasmic reticulum lumen; endoplasmic reticulum membrane; extrinsic to membrane; focal adhesion; plasma membrane. Molecular Function: enzyme binding; protein binding; protein domain specific binding; receptor binding; U-plasminogen activator receptor activity. Biological Process: attachment of GPI anchor to protein; chemotaxis; fibrinolysis; negative regulation of apoptosis; neutrophil degranulation; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of protein amino acid phosphorylation; signal transduction

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425