ELISA/assay

Porcine DMT1 (Divalent metal transporter 1) ELISA Kit

MBS7606135

48-Strip-Wells

300 USD

ELISA Kit

Porcine DMT1 (Divalent metal transporter 1) ELISA Kit

[DMT1 (Divalent metal transporter 1)]

[SLC11A2; DCT1; DMT-1; NRAMP2; Solute carrier family 11 member 2; Divalent cation transporter 1; Natural resistance-associated macrophage protein 2]

[divalent metal transporter 1; Divalent metal transporter 1; natural resistance-associated macrophage protein 2; solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2; Divalent metal transporter 1]

[DMT1]

[slc11a2; slc11a2; DMT1; cb426; fa07b10; wu:fa07b10; zgc:136699]

Porcine

547

This assay has high sensitivity and excellent specificity for detection of DMT1 . No significant cross-reactivity or interference between DMT1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DMT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DMT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DMT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DMT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DMT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DMT1 can be calculated.

27475929

AAO16988.1

60,107 Da

Iron Uptake And Transport Pathway (1114051); Lysosome Pathway (98649); Lysosome Pathway (96865); Metal Ion SLC Transporters Pathway (1114033); SLC-mediated Transmembrane Transport Pathway (1114010); Transmembrane Transport Of Small Molecules Pathway (1114006); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (1114026)

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425