antibody

UBE3A Rabbit Polyclonal

MBS7605422

0.1 mg

290 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

UBE3A Rabbit Polyclonal

[UBE3A]

[ANCR, AS, E6 AP, E6AP, E6AP ubiquitin protein ligase, EPVE6AP, FLJ26981, HPVE6A, UBE3A, ubiquitin protein ligase E3A]

[UBE3A, partial; Ubiquitin-protein ligase E3A; ubiquitin-protein ligase E3A; ubiquitin protein ligase E3A; E6AP ubiquitin-protein ligase; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54]

[UBE3A]

[UBE3A; UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP; E6AP; EPVE6AP; HPVE6A]

UBE3A_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat; other species are not tested.

585

Please decide the specificity by homology

Purity: >=95% as determined by SDS-PAGE. Purification: Immunogen Affinity Purified

Liquid

-20°C for 24 months (Avoid repeated freeze / thaw cycles.)

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200

Immunohistochemistry

SDS-PAGE

Immunogen: Ubiquitin protein ligase E3A. Calculated MW: 110 kDa

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

823671066

AKI70744.1

Q05086

Adaptive Immune System Pathway (1269171); Androgen Receptor Signaling Pathway (198806); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Coregulation Of Androgen Receptor Activity Pathway (138085); Immune System Pathway (1269170); Regulation Of Telomerase Pathway (137987); Tryptophan Metabolism Pathway (198850); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

UBE3A: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator p27Kip1. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Binds UBQLN1 and UBQLN2. Interacts with the 26S proteasome. Interacts with HCV core protein and targets it to degradation. Interacts with the E6 protein of the cancer- associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53/TP53 tumor-suppressor protein for ubiquitin-mediated proteolysis. Interacts with BPY2. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Ubiquitin conjugating system; Ubiquitin ligase. Chromosomal Location of Human Ortholog: 15q11.2. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; ubiquitin-protein ligase activity. Biological Process: androgen receptor signaling pathway; brain development; positive regulation of protein ubiquitination; protein autoubiquitination; proteolysis; regulation of circadian rhythm. Disease: Angelman Syndrome

0.1 mg

290 USD