ELISA/assay

Human Protein kinase C epsilon type ELISA Kit

MBS760530

48-Strip-Wells

295 USD

ELISA Kit

Human Protein kinase C epsilon type ELISA Kit

Protein kinase C epsilon type

PKCepsilon (Protein Kinase C Epsilon )/PRKCE/PKCE/nPKC-epsilon/MGC125656/PKCEMGC125657/protein kinase C epsilon type/protein kinase C; epsilon

protein kinase C epsilon type; Protein kinase C epsilon type; protein kinase C epsilon type; protein kinase C epsilon; nPKC-epsilon

PRKCE

PRKCE; PRKCE; PKCE; nPKC-epsilon; PKCE

KPCE_HUMAN

Human

737

This assay has high sensitivity and excellent specificity for detection of PRKCE . No significant cross-reactivity or interference between PRKCE and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Sensitivity: < 0.938ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PRKCE were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PRKCE were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PRKCE antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PRKCE antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PRKCE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PRKCE can be calculated.

4885563

NP_005391.1

NM_005400.2

83,674 Da

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Aldosterone Synthesis And Secretion Pathway (1272485); Aldosterone Synthesis And Secretion Pathway (1285075); B Cell Receptor Signaling Pathway (198909); CDC42 Signaling Events Pathway (137994); Calcium Regulation In The Cardiac Cell Pathway (198906); DAG And IP3 Signaling Pathway (1269291); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction. Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; PKC family; Eta subfamily. Chromosomal Location of Human Ortholog: 2p21. Cellular Component: cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane. Molecular Function: actin monomer binding; ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; metal ion binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity; receptor activator activity; signal transducer activity. Biological Process: apoptosis; blood coagulation; cell adhesion; cell cycle; cell division; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; lipopolysaccharide-mediated signaling pathway; macrophage activation during immune response; negative regulation of protein ubiquitination; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; phospholipase C activation; platelet activation; positive regulation of actin filament polymerization; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of insulin secretion; positive regulation of lipid catabolic process; positive regulation of MAPKKK cascade; positive regulation of synaptic transmission, GABAergic; protein amino acid phosphorylation; regulation of peptidyl-tyrosine phosphorylation; regulation of release of sequestered calcium ion into cytosol; release of sequestered calcium ion into cytosol; response to morphine; signal transduction

48-Strip-Wells

295 USD

96-Strip-Wells

390