antibody

TFEB Rabbit Polyclonal

MBS7604860

0.1 mg

290 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

Antibody

TFEB Rabbit Polyclonal

[TFEB]

[AlphaTFEB, bHLHe35, TCFEB, TFEB, transcription factor EB]

[transcription factor EB isoform 2; Transcription factor EB; transcription factor EB; transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35]

[TFEB]

[TFEB; TFEB; TCFEB; BHLHE35; ALPHATFEB; BHLHE35; bHLHe35]

TFEB_HUMAN

polyclonal

IgG

rabbit

Human, Mouse, Rat. Other species are not tested.

490

Please decide the specificity by homology

> 95% as determined by SDS-PAGE; Immunogen Affinity Purified

Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3

Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)

ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Recommended dilution: WB : 1:500-1:2000; IP :1:500-1:2000; IHC : 1:50-1:200; IF : 1:20-1:200

Immunohistochemistry

Western Blot

Immunogen: Transcription factor EB

TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa.

429836865

NP_001161299.2

NM_001167827.2

65-70kd

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p21. Cellular Component: cytoplasm; nucleus. Molecular Function: protein dimerization activity; transcription factor activity. Biological Process: adaptive immune response; autophagy; embryonic placenta development; humoral immune response; lysosome organization and biogenesis; positive regulation of autophagy; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent

0.1 mg

290 USD