STIM1 Mouse Monoclonal | MyBioSource MBS7604554 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

STIM1 Mouse Monoclonal

catalog :

MBS7604554

quantity :

0.1 mg

price :

265 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS7604554 (STIM1 Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

image 2 :

Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7604554 (STIM1 Antibody) at dilution of 1:500

product information

catalog number :

MBS7604554

products type :

Antibody

products full name :

STIM1 Mouse Monoclonal

products short name :

[STIM1]

products name syn :

[Sim]

other names :

[STIM1; Stromal interaction molecule 1; stromal interaction molecule 1; stromal interaction molecule 1]

products gene name :

[STIM1]

other gene names :

[STIM1; STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E; ]

uniprot entry name :

STIM1_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

host :

Mouse

reactivity :

Rat, Human, Mouse

sequence length :

685

specificity :

Human, Mouse ,Rat; other species are not tested. Please decide the specificity by homology

purity :

Purity: >=95% as determined by SDS-PAGE. Purification: Protein A+G purification

form :

Liquid

storage stability :

Store at -20°C for 24 months (Avoid repeated freeze/thaw cycles.)

tested application :

ELISA (EIA), Western Blot (WB), Immunohisctochemistry (IHC)

app notes :

WB : 1:200-1:2000. IHC : 1:50-1:500

image1 heading :

Immunohistochemistry

image2 heading :

SDS-PAGE

other info1 :

Immunogen: Stromal interaction molecule 1

other info2 :

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Calculated MW: 77 kD

products description :

Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores (By similarity). Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1 (PubMed:16208375). Involved in enamel formation (By similarity). Activated following interaction with TMEM110/STIMATE, leading to promote STIM1 conformational switch (By similarity).

ncbi gi num :

429142778

ncbi acc num :

AFZ76986.1

uniprot acc num :

P84903

ncbi pathways :

Adaptive Immune System Pathway (1269171); Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway (1269184); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Cardiac Conduction Pathway (1339115); Elevation Of Cytosolic Ca2+ Levels Pathway (1269346); Hemostasis Pathway (1269340); Immune System Pathway (1269170); Ion Homeostasis Pathway (1339121); Muscle Contraction Pathway (1269868)

ncbi summary :

This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

uniprot summary :

STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines. Protein type: Calcium-binding; Endoplasmic reticulum; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to plasma membrane; microtubule; sarcoplasmic reticulum membrane. Molecular Function: calcium channel regulator activity; calcium ion binding; identical protein binding; microtubule plus-end binding; protease binding; protein binding. Biological Process: activation of store-operated calcium channel activity; calcium ion transport; detection of calcium ion; regulation of calcium ion transport. Disease: Immunodeficiency 10; Myopathy, Tubular Aggregate, 1

size1 :

0.1 mg

price1 :

265 USD

more info or order :

MyBioSource product webpage