Human Interleukin-26 ELISA Kit | MyBioSource MBS760353 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interleukin-26 ELISA Kit

catalog :

MBS760353

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS760353

products type :

ELISA Kit

products full name :

Human Interleukin-26 ELISA Kit

products short name :

[Interleukin-26]

products name syn :

[IL26/Interleukin-26/IL-26/Protein AK155/AK155/interleukin 26]

other names :

[interleukin-26; Interleukin-26; interleukin-26; interleukin 26; Protein AK155]

products gene name :

[IL26]

other gene names :

[IL26; IL26; AK155; IL-26; AK155; IL-26]

uniprot entry name :

IL26_HUMAN

reactivity :

Human

sequence length :

171

specificity :

This assay has high sensitivity and excellent specificity for detection of IL26. No significant cross-reactivity or interference between IL26 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1000pg/ml. Sensitivity: <9.375pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL26 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL26 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL26 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL26 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL26 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL26 can be calculated.

ncbi gi num :

8923756

ncbi acc num :

NP_060872.1

ncbi gb acc num :

NM_018402.1

ncbi mol weight :

19,843 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

ncbi summary :

This gene was identified by its overexpression specifically in herpesvirus samimiri-transformed T cells. The encoded protein is a member of the IL10 family of cytokines. It is a secreted protein and may function as a homodimer. This protein is thought to contribute to the transformed phenotype of T cells after infection by herpesvirus samimiri. [provided by RefSeq, Jul 2008]

uniprot summary :

IL26: May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin. Belongs to the IL-10 family. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q15. Cellular Component: cytosol; extracellular space. Molecular Function: cytokine activity. Biological Process: cell-cell signaling; immune response; inflammatory response; negative regulation of epithelial cell proliferation; positive regulation of cytokine secretion; positive regulation of JAK-STAT cascade; positive regulation of protein kinase B signaling cascade; positive regulation of stress-activated MAPK cascade; positive regulation of transcription from RNA polymerase II promoter

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage