ELISA/assay

Human Eosinophil peroxidase ELISA Kit

MBS760340

48-Strip-Wells

300 USD

ELISA Kit

Human Eosinophil peroxidase ELISA Kit

[Eosinophil peroxidase]

[EPX/EPER/EPO/EPP]

[eosinophil peroxidase; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase]

[EPX]

[EPX; EPX; EPO; EPP; EPXD; EPX-PEN; EPER; EPO; EPP; EPO]

PERE_HUMAN

Human

715

This assay has high sensitivity and excellent specificity for detection of Epor. No significant cross-reactivity or interference between Epor and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epor were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epor were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Epor antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Epor antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Epor amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Epor can be calculated.

182146

AAA58458.1

81,040 Da

Asthma Pathway (83120); Asthma Pathway (532)

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Feb 2016]

EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily. Protein type: Oxidoreductase; EC 1.11.1.7. Chromosomal Location of Human Ortholog: 17q23.1. Molecular Function: heme binding; metal ion binding; peroxidase activity. Biological Process: defense response to nematode; hydrogen peroxide catabolic process; negative regulation of interleukin-10 production; negative regulation of interleukin-5 production; positive regulation of interleukin-4 production; response to oxidative stress. Disease: Eosinophil Peroxidase Deficiency

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425