ELISA/assay

Mouse Protein Wnt-3a ELISA Kit

MBS760270

48-Strip-Wells

300 USD

ELISA Kit

Mouse Protein Wnt-3a ELISA Kit

[Protein Wnt-3a]

[Wnt3a/WNT3A/MGC119418/MGC119419/MGC119420/protein Wnt-3a/wingless-type MMTV integration site family; member 3A]

[protein Wnt-3a; Protein Wnt-3a; protein Wnt-3a; wingless-type MMTV integration site family member 3A]

[Wnt3a]

[WNT3A; WNT3A]

WNT3A_HUMAN

Mouse

352

This assay has high sensitivity and excellent specificity for detection of Wnt3a. No significant cross-reactivity or interference between Wnt3a and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 0.312-20ng/ml. Sensitivity: 0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wnt3a were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wnt3a were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Wnt3a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Wnt3a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Wnt3a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Wnt3a can be calculated.

14916475

NP_149122.1

NM_033131.3

42,905 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Canonical Wnt Signaling Pathway (138032); Cardiac Progenitor Differentiation Pathway (712094); Class B/2 (Secretin Family Receptors) Pathway (1269570); DNA Damage Response (only ATM Dependent) Pathway (198827); Disassembly Of The Destruction Complex And Recruitment Of AXIN To The Membrane Pathway (1269601); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855); GPCR Ligand Binding Pathway (1269544)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix. Molecular Function: frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor agonist activity; receptor binding; transcription coactivator activity. Biological Process: axon guidance; cell proliferation in forebrain; cell proliferation in midbrain; dorsoventral neural tube patterning; extracellular matrix organization and biogenesis; heart looping; hemopoiesis; hippocampus development; in utero embryonic development; inner ear morphogenesis; mammary gland development; negative regulation of axon extension involved in axon guidance; negative regulation of fat cell differentiation; negative regulation of neurogenesis; neuron differentiation; osteoblast differentiation; palate development; paraxial mesodermal cell fate commitment; positive regulation of B cell proliferation; positive regulation of caspase activity; positive regulation of cell proliferation; positive regulation of collateral sprouting in the absence of injury; positive regulation of cytokine production; positive regulation of mesodermal cell fate specification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of skeletal muscle development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; signalosome assembly; spinal cord association neuron differentiation; synaptogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; Wnt receptor signaling pathway through beta-catenin

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425