P21; CDKN1A Rabbit Polyclonal | MyBioSource MBS7602300 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

P21; CDKN1A Rabbit Polyclonal

catalog :

MBS7602300

quantity :

0.1 mg

price :

265 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical of paraffin-embedded human kidney using MBS7602300 (P21 antibody) at dilution of 1:50

image 2 :

HEK-293 cells were subjected to SDS PAGE followed by western blot with MBS7602300 (P21 antibody) at dilution of 1:500

product information

catalog number :

MBS7602300

products type :

Antibody

products full name :

P21; CDKN1A Rabbit Polyclonal

products short name :

[P21; CDKN1A]

products name syn :

[CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1]

other names :

[p21; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); CDK-interacting protein 1; Melanoma differentiation-associated protein; p21]

products gene name :

[P21; CDKN1A]

other gene names :

[Cdkn1a; Cdkn1a; P21; CDKI; CIP1; SDI1; Waf1; mda6; CAP20; Cdkn1; p21WAF; p21Cip1; Cip1; Waf1]

uniprot entry name :

CDN1A_MOUSE

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat; other species are not tested.

sequence length :

159

specificity :

Please decide the specificity by homology

purity :

> 95% as determined by SDS-PAGE; Immunogen affinity purified

form :

Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3

storage stability :

Store at -20 for 24 months (Avoid repeated freeze / thaw cycles.)

tested application :

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:200-1:1000. IHC: 1:20-1:200

image1 heading :

Immunohistochemical

image2 heading :

Western Blot

other info1 :

Immunogen: cyclin-dependent kinase inhibitor 1A (p21, Cip1)

products description :

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

ncbi gi num :

50300977

ncbi acc num :

AAT73700.1

uniprot acc num :

P38936

ncbi mol weight :

21kDa

ncbi pathways :

AKT Phosphorylates Targets In The Cytosol Pathway (1366890); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (1367971); Adaptive Immune System Pathway (1367473); Alpha6-Beta4 Integrin Signaling Pathway (198373); Bladder Cancer Pathway (83308); Bladder Cancer Pathway (527); Cell Cycle Pathway (1367897); Cell Cycle Checkpoints Pathway (1367898); Cell Cycle, Mitotic Pathway (1367917); Cell Cycle Pathway (83251)

ncbi summary :

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at the G1 pahse. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

uniprot summary :

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase. Protein type: Cell cycle regulation; Inhibitor. Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; nucleus; perinuclear region of cytoplasm. Molecular Function: cyclin binding; cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase inhibitor activity; metal ion binding; protein binding; protein complex binding; protein kinase inhibitor activity; ubiquitin protein ligase binding. Biological Process: cell cycle; cell cycle arrest; cellular response to amino acid starvation; cellular response to extracellular stimulus; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of phosphorylation; positive regulation of B cell proliferation; positive regulation of fibroblast proliferation; positive regulation of programmed cell death; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of mitotic cell cycle; regulation of protein import into nucleus, translocation; response to DNA damage stimulus; response to UV

size1 :

0.1 mg

price1 :

265 USD

more info or order :

MyBioSource product webpage