Human Neurokinin B ELISA Kit | MyBioSource MBS760185 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Neurokinin B ELISA Kit

catalog :

MBS760185

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS760185

products type :

ELISA Kit

products full name :

Human Neurokinin B ELISA Kit

products short name :

[Neurokinin B]

products name syn :

[NKB]

other names :

[neurokinin B; Tachykinin-3; tachykinin-3; tachykinin 3; ZNEUROK1]

products gene name :

[NKB]

other gene names :

[TAC3; TAC3; NKB; HH10; NKNB; PRO1155; ZNEUROK1; NKNB; NKB]

uniprot entry name :

TKNK_HUMAN

reactivity :

Human

sequence length :

121

specificity :

This assay has high sensitivity and excellent specificity for detection of NKB. No significant cross-reactivity or interference between NKB and analogues is observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Competitive. Detection Range: 15.625-1000pg/ml. Sensitivity: <9.375pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NKB were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NKB were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with NKB. During the reaction, NKB in the sample or standard competes with a fixed amount of NKB on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to NKB. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NKB in the samples is then determined by comparing the O D of the samples to the standard curve.

ncbi gi num :

8572053

ncbi acc num :

AAF76980.1

ncbi mol weight :

11,358 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1269592); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543); Tachykinin Receptors Bind Tachykinins Pathway (1269548)

ncbi summary :

This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded preproprotein is proteolytically processed to generate the mature peptide, which is primarily expressed in the central and peripheral nervous systems and functions as a neurotransmitter. This peptide is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

uniprot summary :

TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: extracellular region; extracellular space. Molecular Function: protein binding; receptor binding. Biological Process: female pregnancy; neuropeptide signaling pathway; tachykinin signaling pathway. Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage