ELISA/assay

Rat Neutrophil Gelatinase Associated Lipocalin ELISA Kit

MBS760137

48-Strip-Wells

300 USD

ELISA Kit

Rat Neutrophil Gelatinase Associated Lipocalin ELISA Kit

[Neutrophil Gelatinase Associated Lipocalin]

[Lipocalin-2/LCN2/NGAL/Siderocalin/24p3/25 kDa alpha-2-microglobulin-related subunit of MMP-9/HNL/LCN2/lipocalin 2/lipocalin 2 (oncogene 24p3)/Lipocalin-2/migration-stimulating factor inhibitor/MSFI/neutrophil gelatinase-associated lipocalin/NGALlipocalin-2/Oncogene 24p3/p25/siderocalin]

[neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25]

[NGAL]

[LCN2; LCN2; p25; 24p3; MSFI; NGAL; HNL; NGAL; NGAL]

NGAL_HUMAN

Rat

198

This assay has high sensitivity and excellent specificity for detection of Lipocalin-2. No significant cross-reactivity or interference between Lipocalin-2 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipocalin-2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipocalin-2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Lipocalin-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Lipocalin-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lipocalin-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Lipocalin-2 can be calculated.

38455402

NP_005555.2

NM_005564.4

22,457 Da

Iron Uptake And Transport Pathway (1269948); Transmembrane Transport Of Small Molecules Pathway (1269903)

This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: cytosol; extracellular region; extracellular space. Molecular Function: iron ion binding; protease binding; protein homodimerization activity; transporter activity. Biological Process: cellular iron ion homeostasis; cellular response to nutrient levels; innate immune response; ion transport; positive regulation of cell projection organization and biogenesis; response to drug; response to herbicide; response to mycotoxin; response to virus; siderophore transport; transmembrane transport

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425