This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human 5-Nucleotidase ELISA Kit
catalog :
MBS760122
quantity :
48-Strip-Wells
price :
300 USD
product information
catalog number :
MBS760122
products type :
ELISA Kit
products full name :
Human 5-Nucleotidase ELISA Kit
products short name :
[5-Nucleotidase]
products name syn :
[5-NT/NT5E/5'-NT/CD73/E5NT/eNT/NTE/eN/NT/NT5/5' nucleotidase (CD73)/CD73 antigen/Ecto-5'-nucleotidase/5'-nucleotidase/Purine 5-Prime-Nucleotidase]
other names :
[5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase; 5' nucleotidase, ecto; Ecto-5'-nucleotidase; CD_antigen: CD73]
products gene name :
[5-NT]
other gene names :
[Nt5e; Nt5e; Nt5; CD73; Nt5; Nte; 5'-NT]
uniprot entry name :
5NTD_RAT
reactivity :
Human
sequence length :
576
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data
image2 heading :
Typical Standard Curve
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 0.313-20ng/ml. Sensitivity: 0.188ng/ml
products description :
Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human 5- NT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human 5-NT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human 5-NT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human 5-NT can be calculated.
ncbi gi num :
148747417
ncbi acc num :
NP_067587.2
ncbi gb acc num :
NM_021576.2
ncbi mol weight :
63,969 Da
ncbi pathways :
Metabolic Pathways (132933); Metabolism Pathway (1333271); Metabolism Of Nucleotides Pathway (1333435); Nicotinate And Nicotinamide Metabolism Pathway (83406); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (1333439); Purine Metabolism Pathway (83337); Purine Metabolism Pathway (1333436); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (1333443)
ncbi summary :
may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]
uniprot summary :
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, integral; Phosphatase (non-protein); Membrane protein, GPI anchor; Nucleotide Metabolism - purine; EC 3.1.3.5; Nucleotide Metabolism - pyrimidine. Cellular Component: cell surface; cytoplasm; integral to membrane; membrane; plasma membrane. Molecular Function: 5'-nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding. Biological Process: adenosine biosynthetic process; adenosine metabolic process; AMP catabolic process; brain development; dephosphorylation; leukocyte adhesion; negative regulation of inflammatory response; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; response to aluminum ion
size1 :
48-Strip-Wells
price1 :
300 USD
size2 :
96-Strip-Wells
price2 :
415
size3 :
5x96-Strip-Wells
price3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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