catalog number :
MBS760115
products type :
ELISA Kit
products full name :
2,3-dinor Thromboxane B2 ELISA Kit
products short name :
[2,3-dinor Thromboxane B2]
products name syn :
[2,3-dinor-TXB2 (2,3-dinor Thromboxane B2)]
products gene name :
[2,3-dinor-TXB2]
specificity :
This assay has high sensitivity and excellent specificity for detection of 2,3-dinor-TXB2. No significant cross-reactivity or interference between 2,3-dinor-TXB2 and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive ELISA, Coated with Antigen. Detection Range: 15.625-1000pg/ml. Sensitivity: 9.375pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2,3- dinor-TXB2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2,3- dinor-TXB2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 2,3-dinor-TXB2. During the reaction, 2,3-dinor-TXB2 in the sample or standard competes with a fixed amount of 2,3-dinor-TXB2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 2,3-dinor-TXB2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 2,3-dinor-TXB2 in the samples is then determined by comparing the OD. of the samples to the standard curve.
size4 :
10x96-Strip-Wells